Composite

Part:BBa_K4653121:Design

Designed by: Tangkun Zhu   Group: iGEM23_SZU-China   (2023-10-10)


SacB promoter - SpoVG RBS - MazE/F - Fd Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 962
    Illegal AgeI site found at 682
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1231
    Illegal SapI.rc site found at 1175


Design Notes

And we needed a terminator to help us stop the above transcription, so we chose the fd terminator, which is the central terminator of bacteriophage fd. Additionally, we repeated the fd terminator as a strong terminator.


Source

The SacB promoter is separated from the B. subtilis. The SpoVG ribosome binding site (RBS) for B. subtilis for MoClo Level 0 with 6bp scar/spacer. The fd terminator is central terminator from bacteriophage fd.

References