Composite
Part:BBa_K4653121:Design
Designed by: Tangkun Zhu Group: iGEM23_SZU-China (2023-10-10)
SacB promoter - SpoVG RBS - MazE/F - Fd Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 962
Illegal AgeI site found at 682 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1231
Illegal SapI.rc site found at 1175
Design Notes
And we needed a terminator to help us stop the above transcription, so we chose the fd terminator, which is the central terminator of bacteriophage fd. Additionally, we repeated the fd terminator as a strong terminator.
Source
The SacB promoter is separated from the B. subtilis. The SpoVG ribosome binding site (RBS) for B. subtilis for MoClo Level 0 with 6bp scar/spacer. The fd terminator is central terminator from bacteriophage fd.